Odorant Binding and Conformational Changes of a Rat Odorant-binding Protein
نویسندگان
چکیده
منابع مشابه
Odorant binding and conformational changes of a rat odorant-binding protein.
Odorant-binding proteins (OBPs) are lipocalins secreted in the nasal mucus of vertebrates, which convey odorants to their neuronal receptors. We compared the binding properties of a recombinant rat OBP (OBP-1F) using a set of six odorants of various chemical structures. We examined the binding properties by both fluorescent probe competition and isothermal titration calorimetry. OBP-1F affinity...
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From a rat olfactory epithelium cDNA library clones encoding a lipocalin were isolated with sequence identity to the previously described salivary-specific alpha-2u globulin and the N-terminal region of mouse odorant-binding proteins OBP-III and OBP-IV. In situ hybridization showed strong expression in nasal glands displaying a pattern equivalent to rat OBP1. Heterologously expressed protein wa...
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1. General features of OBP OBP was first found in 1985 in bovine nasal mucosa as an abundant protein, which is capable of binding bell pepper odor, 2-isobutyl-3-methoxy pyridine. This protein has a hydrophobic internal cavity in the β-barrel domain, and it can bind low molecular weight (usually less than 300 Da) hydrophobic molecules in the cavity with broad specificities. Generally its affinit...
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The stability and functionality of GCC-bOBP, a monomeric triple mutant of bovine odorant binding protein, was investigated, in the presence of denaturant and in acidic pH conditions, by both protein and 1-aminoanthracene ligand fluorescence measurements, and compared to that of both bovine and porcine wild type homologues. Complete reversibility of unfolding was observed, though refolding was c...
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ژورنال
عنوان ژورنال: Chemical Senses
سال: 2004
ISSN: 1464-3553
DOI: 10.1093/chemse/bjh017